Ototoxicity of an association of insecticides compounds containing dichlorvos and cypermethrin in Wistar rats

Aléxia dos Reis; Suelen Pizzolatto Dalmolin; Dora de Athayde Saul; Márcia Salgado Machado; Eliane Dallegrave

doi:10.1590/1982-0216/20222424522

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Ototoxicity of an association of insecticides compounds containing dichlorvos and cypermethrin in Wistar rats

Aléxia dos Reis; Suelen Pizzolatto Dalmolin; Dora de Athayde Saul; Márcia Salgado Machado; Eliane Dallegrave

http://dx.doi.org/10.1590/1982-0216/20222424522 Revista CEFAC, vol.24, n2, e4522, 2022

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Abstract

Purpose: to evaluate possible ototoxicity secondary to exposure to a combination of pesticides (dichlorvos and cypermethrin based insecticides).

Methods: the Wistar rats were divided into 3 groups (12 animals per group): control (water), positive control for hearing damage (cisplatin) and experimental (exposed to dichlorvos and cypermethrin). The amplitude of distortion product otoacoustic emissions was assessed before and after the exposure. Systemic toxicity signs were also evaluated (clinical signs, weight gain and plasma cholinesterase). Wilcoxon test analyzed the post-exposure amplitudes compared to pre-exposure and Kruskal Wallis following Dunn’s post hoc tests analyzed the amplitudes’ variation. Normally distributed variables were evaluated by Student's t test.

Results: body weight and plasma cholinesterase values were similar comparing the pre and post exposure in the experimental group. The control group did not manifest significant amplitude reduction of otoacoustic emissions between the pre and post evaluation. In the group exposed to cisplatin there was a significant reduction in amplitudes at 12 kHz on the right (p = 0.006; Wilcoxon) and 4 kHz on the left (p = 0.032; Wilcoxon). In the group exposed to pesticides, there was a significant reduction in the right ear at 4 kHz (p = 0.034; Wilcoxon) and 8 kHz (p = 0.019; Wilcoxon) and in the left ear at 4 kHz (p = 0.007; Wilcoxon), 6 kHz (p = 0.023; Wilcoxon), 8 kHz (p = 0.045; Wilcoxon) and 12 kHz (p = 0.028; Wilcoxon).

Conclusion: there was ototoxicity in the experimental group, without a relevant systemic toxicity.

Keywords

Ototoxicity, Agrochemicals, Rats, Audiology

Referências

1. Zeigelboim BS, Malisky JS, Rosa MR, Lacerda ABM, Alcaraz PS, Fonseca VR. The importance of otoneurological evaluation in Brazilian workers exposed to pesticides: a preliminary study. Int. Arch. Otorhinolaryngol. 2019;23(4):389-95.

2. Hernández AF, Gil F, Lacasaña M. Toxicological interactions of pesticide mixtures: an update. Arch Toxicol. 2017;91(10):3211-23.

3. Sturza J, Silver MK, Xu L, Li M, Mai X, Xia Y et al. Prenatal exposure to multiple pesticides is associated with auditory brainstem response at 9months in a cohort study of Chinese infants. Environ Int. 2016;92-93:478-85.

4. Kós MI, Hoshino AC, Asmus CIF, Mendonça R, Meyer A. Efeitos da exposição a agrotóxicos sobre o sistema auditivo periférico e central: uma revisão sistemática. Cad Saúde Pública. 2013;29(8):1491-1506.

5. Alcarás PAS, Larcerda ABM, Marques JM. Study of Evoked Otoacoustic Emissions and suppression effect on workers exposed to pesticides and noise. CoDAS. 2013;25(6):527-33.

6. Teixeira CF, Augusto LGS, Morata TC. Saúde auditiva de trabalhadores expostos a ruído e inseticidas. Rev Saúde Pública. 2003;37(4):417-23.

7. Cazé AMB, Lacerda ABM, Lüders D, Conto J, Marques J, Leroux T. Perception of the quality of life of tobacco growers exposed to pesticides: emphasis on health, hearing, and working conditions. Int. Arch. Otorhinolaryngol. 2019;23(1):50-9.

8. Reis AD, Dalmolin SP, Dallegrave E. Animal models for hearing evaluations: a literature review. Rev. CEFAC. 2017;19(3):417-28.

9. Reis AD, Cunha EO, CostaValle MT, Machado MS, Dallegrave E. Effects of subchronic inhalation exposure to an organophosphorus insecticide compound containing dichlorvos on wistar rats' otoacoustic emissions. Braz J Otorhinolaryngol. 2022;88(1):28-35.

10. Cunha EO, Reis AD, Macedo MB, Machado MS, Dallegrave E. Ototoxicity of cypermethrin in Wistar rats. Braz J Otorhinolaryngol. 2019;86(5):587-92.

11. Andrade Filho A, Souza SD. Anticolinesterásicos. In: Andrade Filho A, Campolina D, Dias MB, editors. Toxicologia na prática clínica. 2nd ed. Belo Horizonte: Folium; 2013. p. 89-95.

12. Wakeling EN, Neal AP, Atchison WD. Pyrethroids and their effects on ion channels. In: Soundararajan R, editor. Pesticides - advances in chemical and botanical pesticides [Internet]. London: IntechOpen. 2012. Available from: https://www.intechopen.com/chapters/37969 doi: 10.5772/50330.

13. OECD. Test No. 413: subchronic inhalation toxicity: 90-day study, OECD guidelines for the testing of chemicals, Section 4. 2009. OECD Publishing, Paris.

14. De Freitas MR, Figueiredo AA, Brito GAC, Leitão RFC, Carvalho Junior JV, Gomes Junior RM et al. The role of apoptosis in cisplatin-induced ototoxicity in rats. Braz J Otorhinolaryngol. 2009;75(5):745-52.

15. Rodnitzky RL, Levin HS, Mick DL. Occupational exposure to organophosphate pesticides. Archives of Environmental Health: An International Journal. 1975;30:2:98-103.

16. Sena TRR, Dourado SSF, Lima LV, Antoniolli ÂR. The hearing of rural workers exposed to noise and pesticides. Noise Health. 2018;20(92):23-6.

17. Tomiazzi JS, Pereira DR, Judai MA, Antunes PA, Favareto APA. Performance of machine-learning algorithms to pattern recognition and classification of hearing impairment in Brazilian farmers exposed to pesticide and/or cigarette smoke. Environ Sci Pollut Res Int. 2019;26(7):6481-91.

18. Medithi S, Jonnalagadda PR, Jee B. Predominant role of antioxidants in ameliorating the oxidative stress induced by pesticides. Arch Environ Occup Health. 2021;76(2):61-74.

19. How V, Hashim Z, Ismail P, Omar D, Said SMD, Tamrin SBM. Characterization of risk factors for DNA damage among paddy farm worker exposed to mixtures of organophosphates. Arch Environ Occup Health. 2015;70(2):102-90.

20. Schacht J, Talaska AE, Rybak LP. Cisplatin and aminoglycoside antibiotics: hearing loss and its prevention. Anat Rec (Hoboken). 2012;295(11):1837-50.

21. Lee JN, Kim SG, Lim JY, Dutta RK, Kim SJ, Choe SK et al. 3-Aminotriazole protects from CoCl2-induced ototoxicity by inhibiting the generation of reactive oxygen species and proinflammatory cytokines in mice. Arch Toxicol. 2016;90(4):781-91.

22. Jiang P, Ray A, Rybak LP, Brenner MJ. Role of STAT1 and oxidative stress in gentamicin-induced hair cell death in organ of corti. Otol Neurotol. 2016;37(9):1449-56.

23. Kim YJ, Tian C, Kim J, Shin B, Choo OS, Kim YS et al. Autophagic flux, a possible mechanism for delayed gentamicin-induced ototoxicity. Sci Rep. 2017;7:41356.

24. Choi MJ, Kang H, Lee YY, Choo OS, Jang JH, Park SH et al. Cisplatin-induced ototoxicity in rats is driven by RIP3-dependent necroptosis. Cells. 2019;8(5):409.

Submetido em:
07/08/2022

Aceito em:
28/09/2022

Ototoxicity of an association of insecticides compounds containing dichlorvos and cypermethrin in Wistar rats

Aléxia dos Reis; Suelen Pizzolatto Dalmolin; Dora de Athayde Saul; Márcia Salgado Machado; Eliane Dallegrave

Abstract

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